diff --git a/docker/docker-compose-template.yaml b/docker/docker-compose-template.yaml
index beb9a2ea75..a650cb71ba 100644
--- a/docker/docker-compose-template.yaml
+++ b/docker/docker-compose-template.yaml
@@ -175,8 +175,6 @@ services:
     profiles:
       - mysql
     restart: always
-    env_file:
-      - ./middleware.env
     environment:
       MYSQL_ROOT_PASSWORD: ${MYSQL_PASSWORD:-difyai123456}
       MYSQL_DATABASE: ${MYSQL_DATABASE:-dify}
diff --git a/docker/docker-compose.yaml b/docker/docker-compose.yaml
index 081e438cd3..e5bb8fd9dc 100644
--- a/docker/docker-compose.yaml
+++ b/docker/docker-compose.yaml
@@ -808,8 +808,6 @@ services:
     profiles:
       - mysql
     restart: always
-    env_file:
-      - ./middleware.env
     environment:
       MYSQL_ROOT_PASSWORD: ${MYSQL_PASSWORD:-difyai123456}
       MYSQL_DATABASE: ${MYSQL_DATABASE:-dify}